Research Assistant I- Lee Lab

We are looking for a highly motivated and organized Research Assistant interested in human genetic variation and disease at Boston Children's Hospital. This position will involve extracting, amplifying, and sequencing genetic material from bulk and single cell tissue samples related to multiple diseases, including neurodegenerative diseases. Training in computational and bioinformatic research methods is also available, if desired. As an NIH funded lab at Boston Children's Hospital, the goal of the Lee lab is to characterize genomic variation and functional change associated with human disease using computational genomics and bioinformatic approaches. Currently, we focus on investigating aberrations in transposable elements, and other types of genomic variants in various human diseases.

This Research Assistant I will be responsible for:

• Understanding and performing various molecular biology protocols, and collecting, processing, and analyzing data.
• Constructing DNA/RNA next generation sequencing libraries.
• Performing experimental validation for genetic variants.
• Maintaining and organizing laboratory records and equipment.
• Performing general lab maintenance and administration.

In order to qualify, you must have:

• Bachelor's degree in Biology, STEM or Psychology
• Experience in RNA isolation from small starting material/small number of cells are preferred.
• Experience with PCR techniques (general PCR, RT-PCR, qRT-PCR, and droplet digital PCR) is highly preferred.
• Experience with cell isolation using FACS would be considered an asset.
• Good problem-solving, written, and oral communication skills.
• Excellent organizational skills.
• Ability to work independently and as part of a team.

Boston Children's Hospital offers competitive compensation and unmatched benefits including flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.